Journal: Frontiers in Physiology
Article Title: Characteristics and transcriptomic analysis of scar tissues on the inner uterine cavity wall in patients with intrauterine adhesions
doi: 10.3389/fphys.2022.990009
Figure Lengend Snippet: Pathological and immunohistochemical results between the control and the experimental groups. (A) . The HE staining (400ⅹ). (B) . The Masson staining (400ⅹ), the fibrosis area was calculated by ImageJ software, and there was significant statistical difference between the two groups (** p < .01). (C) . Immunohistochemical staining of smooth muscle specific marker α -SMA was compared between the two groups.
Article Snippet: The tissue sections were deparaffinized, treated with 3% H2O2 for 25 min to inactivate endogenous peroxidases, heated in 10-mM citrate buffer at 121°C for 30 min for antigen retrieval, blocked in 5% normal serum for 20 min, and incubated with a primary polyclonal rabbit anti-rat antibody specific for α -SMA (1:200 in PBS, Servicebio; GB111364) overnight at 4°C.
Techniques: Immunohistochemical staining, Control, Staining, Software, Marker